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Sharma, Roopali
- Evaluation of Fungal and Bacterial Antagonists for Managing Phytopathogen Fusarium moniliforme var. subglutinans Sheldon, Causing Pokkah Boeng Disease of Sugarcane
Abstract Views :276 |
PDF Views:202
Authors
Anshul Arya
1,
Roopali Sharma
1,
Geeta Sharma
1,
Bhupesh Chandra Kabdwal
1,
Archana Negi
1,
Bhavya Mishra
1
Affiliations
1 Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar – 263145, Uttarakhand, IN
1 Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar – 263145, Uttarakhand, IN
Source
Journal of Biological Control, Vol 31, No 4 (2017), Pagination: 217-222Abstract
In the present investigation potential fungal and bacterial antagonists were tested under laboratory and field conditions against the phytopathogen Fusarium moniliforme var. subglutinans Sheldon, causing Pokkah Boeng disease of sugarcane. Different strains of fungal anatagonists viz; Trichoderma spp. (strains TCMS36, TCMS43, Th3, TCMS9, Th14) and bacterial antagonists viz; Pseudomonas fluorescens (strains Psf173 and Psf02) and Bacillus sp. were tested against the pathogen under in vitro conditions by employing dual culture technique and volatile assay. The results from dual culture revealed that TCMS36 showed the maximum radial growth inhibition i.e., 73.33 per cent followed by Th14 (69.01%), Psf02 (68.33%), Th3(66.04%),TCMS43 (64.7%), Bacillus sp. (63.95%) and TCMS9(63.74%). Volatile assay depicted that the maximum radial growth inhibition was found in TCMS36(70.01%) followed by TCMS9 (60.10%), Th3 (57.25%), Th14 (55.36%), Psf02 (50.83%) and Bacillus sp. (51.79%), while minimum growth inhibition was observed in Psf173 (40.49%). Further studies were conducted in field, where, Th 14, Psf02 and the consortium of both (Th 14+Psf 02) were tested through sett treatment. The minimum disease incidence was observed in the treatment where setts were treated with P. fluorescens (Psf02) followed by consortium (Th 14+Psf 02) and Th 14 i.e. 0.92%, 1.51% and 5.53%, respectively. The findings from research conclude that the fungal and bacterial antagonist strains can exert an inhibitory effect on the radial growth of Fusarium sp. as well as reduce the disease incidence in field. Application of these antagonists in field could be a better management tool without having to depend on hazardous chemicals.Keywords
Bacillus sp., Fusarium sp., Pokkah Boeng, Pseudomonas spp., Trichoderma spp.Full Text
References
- Akrami M, Khiavi HK, Shikhlinski H and Khoshvaghtei H. 2012. Bio controlling two pathogens of chickpea Fusarium solani and Fusarium oxysporum by different combinations of Trichoderma harzianum, Trichoderma asperellum and Trichoderma virens under field conditions. Int J Agri Sci Res. 1(3): 41–45.
- Brunner K, Peterbauer CK, Mach RL, Lorito M, Zeilinger S and Kubicek CP. 2003. The Nacetylglucosaminidase of Trichoderma atroviride is essential for chitinase induction by chitin of and major relevance to bio-control. Curr Gen. 43: 289–295. Crossref. PMid:12748812
- Dal Bello GM, Mónaco CI and Cháves AR. 1997. Study of the effect of volatile metabolites of Trichoderma hamatum on the growth of phytopathogenic soil borne fungi. Rev Iberoam Micol. 14(3):131–4. PMid:17655390
- Duttamajumdar SK. 2004. Bacterial diseases of sugarcane in india: a bird’s eye view. In: Rao GP, Saumtally AS, Rott P, (eds). Sugarcane pathology: bacterial and nematodes diseases, Science Publishers. pp. 15–50.
- Khan MR, Khan SM and Mohiddin FA. 2004. Biological control of Fusarium wilt of chickpea through seed treatment with the commercial formulation of Trichoderma harzianum and/or Pseudomonas fluorescens. Phytopatho Med. 43: 20–25.
- Korsten L, De Jager ES, Paul I, Obagwu J and El-Ghaouth A. 2000. Alternative control of citrus postharvest diseases. Proc Int Soc Citriculture in press.
- Kullnig C, Mach RL, Lorito M and Kubicek CP. 2000. Enzyme diffusion from Trichoderma atroviride to Rhizoctonia solani is a prerequisite for triggering of Trichodermaech 42 gene expression before mycoparasitic contact. Appl Environ. Microbiol. 66: 2232–2234. Crossref. PMid:10788407 PMCid:PMC101480
- Martin JP, Hong HL and Wismer CA. 1961. The corresponding heritability estimate for Pokkah boeng. In Sugar-cane diseases of the world. Vol. 1, Elsevier Publ. Co. New York. 542 p.
- Mohamedy RSR and Alla MAA. 2013. Bio-priming seed treatment for biological control of soil borne fungi causing ischolar_main rot of green bean (Phaseolus vulgaris L.). J Agri Tech. 9(3): 589–599.
- Negi DS, Sharma PK and Gupta RK. 2014. Management of ischolar_main-rot complex disease and assessment of plant growth promoting characters in vegetable pea with native and commercial antagonists through seed biopriming. Int J Rec Sci 5(8): 1416–1421.
- Papavizas GC. 1985. Trichoderma and Gliocladium: Biology, ecology, and potential for biocontrol. Ann Rev Phytppath. 23: 23-54. Crossref.
- Raza W, Faheem M, Yousaf S, Rajer FU and Yameen M. 2013. Volatile and non-volatile antifungal compounds produced by Trichoderma harzianum SQR-T037 suppressed the growth of Fusarium oxysporum f. sp. niveum. Sci Lett. 1(1): 21–24.
- Sharfuddin C and Mohanka R. 2012. In vitro antagonism of indigenous Trichoderma isolates against phytopathogen causing wilt of lentil. Int J L Sci Pharma Res. 2(3): 195–202.
- Sivasithamparam K and Ghisalberti EL. 1998. Secondar metabolism in Trichoderma and Gliocladium, In: Kubicek CP, Harman GE, and Ondik KL (Eds). Trichoderma and Gliocladium: basic biology, taxonomy and genetics. Taylor and Francis, pp. 139–191.
- Sundaramoorthy S and Balabaskar P. 2013. Trichoderma spp. against wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici. J App Bio Biotec. 1(03): 36–40.
- Vincent JM. 1947. Distortions of fungal hyphae in the presence of certain inhibitors. Phytopath. 48: 268–270. Crossref.
- Vishwakarma SK, Kumar PL, Nigam A, Singh A and Kumar A. 2013. Pokkah Boeng: An emerging disease of sugarcane. J Pl Patho Microbio. 4(3): 1–5.
- Wakker JH and Went FAF C. 1896. Overzicht van de Ziekten van het suikerriet op Java.
- Zeininger S, Galhaup C, Payer K, Woo SL, Mach RL, Fekete C, Lorito M and Kubicek CP. 1999. Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fun Genet Biol. 26:131–140. Crossref. PMid:10328983
- Bio-Intensive Management of Major Soil Borne Diseases of Tomato In Uttarakhand
Abstract Views :239 |
PDF Views:220
Authors
Affiliations
1 Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar – 263145, IN
1 Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar – 263145, IN
Source
Journal of Biological Control, Vol 33, No 4 (2019), Pagination: 353-359Abstract
A glasshouse and field experiments were conducted by using Trichoderma harzianum (Th 43), Pseudomonas spp. (Pf 173) and Jas Mycorrhizal (AMF) for plant growth promotion and management of ischolar_main rot and wilt disease of tomato. There were eight treatment combinations for glass house as well as field experiment. In glasshouse, maximum germination (91%), shoot and ischolar_main length (40.83 cm, 9.79 cm), fresh and dry shoot weight (152.00 g, 28.38 g), fresh and dry ischolar_main weight (9.33 g, 3.90 g), plant vigour index (4301.45) respectively was recorded in treatment combination of Th 43 + Pf 173 + AMF. In experimental field, maximum reduction of disease over control (72.16%) was found in treatment combination of T. harzianum (Th 43) + Pseudomonas spp. (Pf 173) + Jas Mycorrhiza (AMF). The maximum yield of tomato (486.70 q/ha) was also recorded in same treatment combination.Keywords
Growth Promotion, Jas Mycorrhizal, Pseudomonas spp., Trichoderma harzianumFull Text
References
- Agrios GN. 2005. Plant Pathology. USA: Academic press. Abhilash PC, Singh N. 2009. Pesticides use and application: An Indian scenario. J. Hazard Mater. 165: 1-12. https:// doi.org/10.1016/j.jhazmat.2008.10.061 PMid:19081675
- Akkopru, Demir. 2005. Biological control of fusarium wilt in tomato caused by Fusarium oxysporum f. sp. lycopersici by AMF glomus intraradices and some Rhizobacteria. J Phytopathol. 153: 544-550. https://doi.org/10.1111/j.1439-0434.2005.01018.x
- Azarmi R, Hajieghrari B, Giglou A. 2011. Effect of Trichoderma isolates on tomato seedling growth response and nutrient uptake. Afr J Biotechnol. 10: 5850-5855.
- Bonfante P, Genre A. 2010. Mechanisms underlying beneficial plant- fungus interactions in mycorrhizal symbiosis. Nat Commun. 1: 48. https://doi.org/10.1038/ncomms1046 PMid:20975705
- Elad Y, Zaqs ZY, Zuriel S, Chet I. 2007. Use of Trichoderma harzianum or alternation with fungicides to control cucumber grey mould (Botrytis cinerea) under commercial green house conditions. Can J Bot. 42(3): 324-332.
- Glick BR. 1995. Enhancement of plant growth by free living bacteria. Can J Microbiol. 41: 109-117.
- https://doi.org/10.1139/m95-015
- Harman GE. 2000. Myths and dogmas of biocontrol: Changes in perceptions derived from research on Trichoderma harzianum T- 22. Plant Dis. 84: 377-393. https://doi.org/10.1094/PDIS.2000.84.4.377
- Kloepper JW, Scher FM, Laliberti M, Tipping B. 1986. Emergence promoting bacteria: Description and implication for agriculture. pp. 155-164. In: Swinburne TR (Ed.). Iron Siderophore and Plant Disease. Planum, New York. https://doi.org/10.1007/978-1-4615-94802_17
- Leoz BM, Garbisu C, Charcosset YJ, Perez, Jose MS, Antigueded I and Romera ER. 2013. Non target effect of these formulated pesticides on microbially mediated processes in a clay loom soil. Sci Total Environ. 449c: 345-354 https://doi.org/10.1016/j.scitotenv.2013.01.079 PMid:23454695
- Mwangi MW, Monda EO, Sheila AO, Jefwa JM. 2011. Inoculation of tomato seedlings with Trichoderma harzianum and arbuscular mycorrhizal fungi and their effect on growth and control of wilt in tomato seedlings. Braz J Microbiol. 42: 508-513. https://doi.org/10.1590/S1517-83822011000200015 PMid:24031662 PMC id:PMC3769820 National Horticulture Board of India, data base 2013.
- Robert RW. 2005. Growing tomatoes. University of Georgia College of Agricultural and Environmental Sciences. Bulletin. 1271.
- Shoresh M, Harman GE. 2008. The molecular basis of shoot responses of maize seedlings to Trichoderma harzianum T22 inoculation of the ischolar_main: A proteomic approach. Plant Physiol. 147: 2147-2163. https://doi.org/10.1104/pp.108.123810
- Singh SP, Singh HB, Singh DK. 2013. Trichoderma harzianum and Pseudomonas spp. mediated management of Sclerotium rolfsii rot in tomato (Lycopersicon esculentum Mill.). Bioscan. 8(3): 801-804.
- Srivastava R, Khalid A, Singh US, Sharma AK. 2010. Evaluation of arbuscular mycorrhizal fungus, fluorescent pseudomonas and Trichoderma harzianum formulation against Fusarium oxysporum f. sp. lycopersici for the management of tomato wilt. Biol Control. 53: 24-31. https://doi.org/10.1016/j.biocontrol.2009.11.012
- Tanwar A, Kadian A, Gupta A. 2013. Arbuscular mycorrhizal inoculation and super phosphate application influence plant growth and yield of Capsicum annuum. J Soil Sci Plant Nutrition. 13(1): 55-66. https://doi.org/10.4067/S0718-95162013005000006
- Waiganjo MM, Wabule NM, Nyongesa D, Kibaki JM, Onyango I, Wepukhulu SB, Muthaka NM. 2006. Tomato production in Kirinyaga district Kenya, a baseline survey report. KARI/IPM CRSP, Nairobi, Kenya. pp. 1-43.
- Wang B, Qiu YL. 2006. Phylogenetic distribution and evolution of mycorrhizas in land plants. Mycorrhiza 16: 299-363. https://doi.org/10.1007/s00572-005-0033-6 PMid:16845554
- Watterson JC. 1986. Diseases of the tomato crops. Champan and Hall Ltd. Ny. pp. 461-462. PMCid:PMC1202784
- Yigit F and Dikilitas M. 2007. Control of Fusarium wilt of tomato by combination of fluorescent pseudomonas, non-pathogen Fusarium and Trichoderma harzianum T-22 in greenhouse conditions. Plant Pathol J. 6(2): 159-163. https://doi.org/10.3923/ppj.2007.159.163
- Evaluation of Bioagents for their Compatibility in the Development of Consortium for Enhanced Efficacy
Abstract Views :244 |
PDF Views:119
Authors
Affiliations
1 Department Plant Pathology, College of Agriculture, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar - 263145, Uttarakhand, IN
1 Department Plant Pathology, College of Agriculture, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar - 263145, Uttarakhand, IN
Source
Journal of Biological Control, Vol 34, No 2 (2020), Pagination: 164-167Abstract
The concept of development of microbial consortia for bio-control relies on the fact that bioagents under natural habitats live in communities with some benefits for plants. Application of bioagents in a consortium may improve efficacy, reliability and consistency of the bioagents even under diverse soil and environmental conditions. Diversity in biocontrol mechanisms offered by each bioagent in consortium may help in enhancing disease suppressiveness and may also strengthen the capabilities of the partners in an additive or synergistic manner. Till now no guideline has been published for the evaluation of bioagents to test their compatibility before developing bioagent consortium. In the present studies compatibility among biocontrol potential Trichoderma-Pseudomonas and Trichoderma-Trichoderma isolates was studied by dual culture, mixed formulations and using cell free cultures. In dual culture all the combinations (14 no.) were found compatible with each other as no isolate inhibited the growth of one-another i.e. absence of inhibition zone. All the mixed formulations of potential Trichoderma-Pseudomonas isolates (8 no.) were found compatible with each other as they were growing simultaneously on PDA without antagonizing the growth of other or formation of inhibition zone in their combinations. The cell free cultures of each Trichoderma and Pseudomonas isolates tested with each other using Food Poison Technique showed synergistic effects on their fresh mycelial weight among some combinations while majority showed no significant differences with their checks. Further all the combinations (14 no.) were tested for their effects on seed germination and vigour index of chickpea in glasshouse. All the combinations showed significantly better seed germination while some combinations viz. Th14+Psf173, TCMS36+Psf173,Th17+Th19,Th17+Psf2,Th17+TCMS36 and Th14+Psf2 showed better plant vigour index (43.5 to 44.9% ) as compared to their checks (28.8 to 41.5%).These guidelines could be used before developing bioagent consortium and evaluation in field for crop health management.
Keywords
Chickpea, Microbial Consortia, Trichoderma harzianum, Pseudomonas fluorescens.Full Text
References
- Arras G, Arru S. 1997. Mechanism of action of some microbial antagonists against fungal pathogens. Ann Microbiol Enzymol. 47: 97-120.
- Dandurand LM, Knudsen GR. 1993. Influence of Pseudomonas flourescens on hyphal growth and biocontrol, activity of Trichoderma harzianum in the spermosphere of pea. Phytopathol. 83: 265-270. https://doi.org/10.1094/Phyto-83-265
- Elad Y, Freeman S. 2002. Biological control of fungal plant pathogens. In: The Mycota XI: Agricultural Applications. Kempken, Springer-Verlag, Berlin, Germany
- Hubbard JP, Harman GE, Hadar Y. 1983. Effect of soil borne. Pseudomonas spp. on the biological control agent, Trichoderma hamatum. on pea seeds. Phytopathology 73: 655-659.https://doi.org/10.1094/Phyto-73-655
- Jhumishree M, Singh SN, Sonkar SS. 2018. Growth promotion of chickpea plant on treatment with native isolates of Trichoderma spp. J Pharmacog Phytochem. 7: 1631-1636
- Kharb RPS, Lather BPS, Deswal DP. 1994. Prediction of field emergence through heritability and genetic advance of vigour parameters. Seed Sci Technol. 82: 461-466.
- Manjula K, Krishna Kishore G, Girish AG, Singh SD. 2004.Combined application of Pseudomonas fluorescens and Trichoderma viride has an improved biocontrol activity against stem rot in groundnut. Plant Pathol J. 20: 75-80. https://doi.org/10.5423/PPJ.2004.20.1.075
- Raupach GS, Kloepper JW. 1998. Mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology 88: 1158-1164. https://doi.org/10.1094/PHYTO.1998.88.11.1158 PMid:18944848
- Rini CR, Sulochana KK. 2007. Usefulness of Trichoderma and Pseudomonas against Rhizoctonia solani and Fusarium oxysporum infecting tomato. J Trop Agric.45:21–28.
- Sharma T, Navin K, Nishant R. 2012. Isolation, screening and characterization of PGPR isolates from rhizosphere of rice plants in Kashipur region (Tarai region). Biotechnol Int. 5: 69–84.
- Sivakumar D, Wijeratnam RSW, Wijesundera RLC, Marikar FMT, Abeyesekere M. 2000. Antagonistic effect of Trichoderma harzianum on post harvest of Rambutan, (Nephelium lappaceum) Phytoparasitica 28: 240247. https://doi.org/10.1007/BF02981802 https://doi.org/10.1007/BF02981802
- Thakkar A, Saraf M. 2015. Development of microbial consortia as a biocontrol agent for effective management of fungal diseases in Glycine max L. Arch Phytopathol Plant Prot. 48: 459-474. https://doi.org/10.1080/03235 408.2014.893638